- 背景資料
棉子糖(raffinose)、水蘇糖(stachyose)、毛蕊花糖(verbascose)為”棉子糖系列寡醣”（oligosaccharides in raffinose series）的其中一種。其結構主要由蔗糖以α(1→6)醣苷鍵鍵結半乳糖所構成。
棉子糖(raffinose)➡️三糖➡️半乳糖α(1→6)葡萄糖α(1→2)果糖
水蘇糖(stachyose)➡️四糖➡️半乳糖α(1→6)半乳糖α(1→6)葡萄糖α(1→2)果糖
毛蕊花糖(verbascose)➡️五糖➡️半乳糖α(1→6)半乳糖α(1→6)半乳糖α(1→6)葡萄糖α(1→2)果糖
人體的腸道因無法分泌α-半乳糖苷酶(α-galactosidase)，故無法完全分解具有α-半乳糖苷寡醣之物質(例如:棉子糖、水蘇糖、毛蕊花糖...等物質)，此類物質進入人體之後，最終則會被人體的腸內菌所利用，進而產生發酵作用，部分腸內菌發酵時可能會有氣體產生，故會導致脹氣、腹痛等身體不適症狀。此外，因部分的寡醣具有促進益生菌生長、抑制壞菌數量...等功能，故被稱為益生元 (prebiotics)。
- 操作流程(ASSAY PROCEDURE)
Enzyme Inactivation and Sugar Extraction:
1. Accurately weigh 0.50 ± 0.01 g of flour sample into a glass test-tube (18 x 150 mm) and add 5 mL of ethanol (95% v/v).
2. Incubate the tube in a water bath at 84-88°C for 5 min (this treatment inactivates endogenous enzymes).
3. Quantitatively transfer the tube contents to a 50 mL volumetric flask and adjust the volume to the mark with buffer 1. Allow the sample to extract over 15 min and then mix thoroughly.
4. Transfer 5 mL of this solution/slurry to a glass test-tube (16 x 120 mm).
5. Add 2 mL of chloroform to this solution, mix vigorously on a vortex mixer for 15 sec and centrifuge (1,000 g) for 10 min.
NOTE: This treatment removes most of the lipids from the aqueous upper phase. Insoluble plant material tends to concentrate between the phases.
6. Use the upper aqueous solution (solution A) directly for analysis.
Assay for Glucose, Sucrose and Raffinose-Series Oligosaccharides:
商品特色
商品規格
- 商品規格 120 檢測反應
Bottle 1:
α-Galactosidase suspension (A. niger; 2 mL) in ammonium sulphate.
Store at 4°C. See individual label for expiry date.
Bottle 2:
Invertase solution (yeast; 6 mL) containing sodium azide (0.02%) as a preservative.
Store at 4°C. See individual label for expiry date.
Bottle 3:
GOPOD Reagent Buffer. Buffer (50 mL, pH 7.4), p-hydroxybenzoic acid and sodium azide (0.09% w/v).
Store at 4°C. See individual label for expiry date.
Bottle 4:
GOPOD Reagent Enzymes. Glucose oxidase plus peroxidase and 4aminoantipyrine. Freeze-dried powder.
Store below -10°C. See individual label for expiry date.
Bottle 5:
D-Glucose standard solution (5 mL, 1.0 mg/mL) in 0.2% (w/v) benzoic acid.
Store sealed at room temperature. See individual label for expiry date.
Bottle 6:
Soy-Flour Reference Sample (containing glucose, sucrose and galactosyl-sucrose oligosaccharides).
Store sealed at room temperature. See individual label for expiry date.
