- 手動檢測流程(MANUAL ASSAY PROCEDURE)
- 自動分析儀檢測流程(AUTO-ANALYSER ASSAY PROCEDURE)
- 微量多孔盤檢測流程 (MICROPLATE ASSAY PROCEDURE)
- 樣品準備(SAMPLE PREPARATION)
1. 樣品稀釋(Sample dilution)
2. 樣品澄清(Sample clarification)
3. 一般考慮因素(General considerations)
- 樣品準備範例(SAMPLE PREPARATION EXAMPLES)
(a) Determination of D-glucose in preserves and other vegetable and fruit products.
Homogenise approx. 10 g of sample in a mixer. Accurately weigh approx. 0.5 g into a 100 mL volumetric flask, mix with 50 mL of distilled water to dissolve, make up to the mark, mix and filter. Discard the first 5 mL of the filtrate. Use the clear, undiluted filtrate for the assay (0.1-2.0 mL).
(b) Determination of D-glucose in milk.
Pipette 20 mL of milk into a 100 mL volumetric flask, add the following solutions and mix after each addition: 10 mL of Carrez I solution, 10 mL of Carrez II solution and 20 mL of NaOH solution (100 mM). Fill up to the mark with distilled water, mix and filter. Use 1.00 mL of the filtrate for the assay.
(c) Determination of D-glucose in fermentation samples and cell culture medium.
Incubate an aliquot (approx. 10 mL) of the solution at approx. 90-95°C for 10 min to inactivate enzyme activity. Centrifuge or filter and use the supernatant or clear filtrate (diluted according to the dilution table, if necessary) for the assay. Alternatively, deproteinisation can be performed with Carrez reagents. Homogenise gelatinous agar media with water and treat further as described above.
(d) Solid foodstuffs.
Mill plant materials to pass a 0.5 mm screen. Homogenise solid foodstuffs such as bread, pastries, chocolate confectionery or candy in a mixer, meat grinder or mortar. Weigh out a representative sample and extract with water (heated to 60°C, if necessary). Quantitatively transfer to a volumetric flask and dilute to the mark with distilled water. Mix, filter and use the appropriately diluted, clear solution for the assay.
商品特色
商品規格
- 商品規格
1️⃣manual format: 110/220 assays
2️⃣auto-analyser format:1000/2000 assays
3️⃣microplate format:1100/2200 assays
- 110 Assays per Kit (cat. no. K-GLUHK-110A)
Bottle 1:
Buffer (25 mL, pH 7.6) plus sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2:
NADP+ plus ATP.
Stable for > 5 years below -10°C.
Bottle 3:
Hexokinase plus glucose-6-phosphate dehydrogenase suspension, 2.25 mL.
Stable for > 2 years at 4°C.
Bottle 4:
D-Glucose standard solution (5 mL, 0.4 mg/mL).
Stable for > 2 years; store sealed at 4°C.
- 220 Assays per Kit (cat. no. K-GLUHK-220A)
This has the same components as the 110 determinations kit, except that it contains two of bottle 2 and two of bottle 3.
