- 成分
Pancreatic Digest of Gelatin | 20.0 g/L |
Magnesium Chloride | 1.4 g/L |
Dipotassium Sulfate | 10.0 g/L |
Cetrimide | 0.3 g/L |
Agar | 13.6 g/L |
pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
胰化明膠(pancreatic digest of gelatin)➡️當作氮源
甘油(glycerol)➡️當作碳源
溴化十六烷基三甲銨(cetrimide)➡️抑菌物質,可抑制多種革蘭氏陽性菌和部分陰性菌
氯化鎂(MgCl2)、硫酸鉀(K2SO4)➡️可促使綠膿桿菌產生毒素和色素,並於此培養基中產生綠色的典型菌落
- 配置方法
1. Suspend 45.3 grams of the medium in 1 liter of purified water.
2. Add 10mL of Glycerol.
3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
4. Autoclave at 121°C for 15 minutes.
5. Cool to 45-50°C.