AFLATOXIN M1(黃麴毒素M1)-定量-快篩試紙-53~150 ppt

• 原理
  側流體免疫層析法 (lateral flow immunochromatographic assay)，為快篩試劑的使用原理，藉由抗體抗原間的專一性與免疫親和力做檢測，被普遍應用在、病毒感染確認、妊娠測試、警局臨檢驗毒等方面。此方法多用於定性檢測，且顯色標定物上也有許多不同的種類，目前大多數的顯色方式是利用抗體吸附在膠體金（colloid gold）來呈色。
  
  AFLATOXIN M1(黃麴毒素M1)，為黃麴毒素B1的羥基化代謝物，可能在牛奶或相關乳製品(例如:嬰兒奶粉)中被發現。當乳牛攝取含有黃麴毒素B1的飼料，瘤胃會對黃麴毒素B1進行吸收，並於肝臟中轉換為黃麴毒素 M1，後續經乳牛代謝後，黃麴毒素M1會存在於乳汁當中。雖然黃麴毒素M1毒性較弱(B1毒性較強)，但長期食用含有黃麴毒素M1的乳製品，依舊會造成嚴重的身體危害
   
• 結果判讀
  極限:
  偵測極限(limit of detection, LOD)： 21 ppt
  定量極限(limit of quantification, LOQ)：53 ppt
  偵測範圍:53–150 ppt
• 操作流程(TEST PROCEDURE)
  1. Insert the test strip into the Raptor cartridge in any of the available slots.
  2. Insert the cartridge containing the test strip into any of the 3 ports within the Raptor Integrated Analysis Platform.
  a. The bar code on the test device will be read. If the QR code for the lot of devices has not been entered into the Raptor, the bar code reader in the front of the Raptor will turn on automatically. Scan the QR code found on the container storing the test devices.
  b. The assay temperature for the Reveal Q+ HS for Aflatoxin M1 test is 65°C. This temperature is programmed  in the Raptor.
  c. When the cartridge is inserted into one of the 3 ports, the port will automatically begin to adjust to the  proper temperature.
  d. The user will not be able to proceed until the incubator temperature reaches 65°C.
  3. Enter the sample ID by either scanning or using the on-screen keyboard.
  4. Mix the milk sample prior to adding the sample into the cartridge.
  5. Pipette 0.4 mL milk sample into the sample port located in the back of the cartridge. Press next. The analysis will take place automatically.
  6. Insert the used pipette into the sample port located in the back of the cartridge. This will prevent double loading the same sample or loading a second sample into the same cartridge.
  7. After a 5 minute incubation, the results will appear on the Raptor screen.

• 產品說明書：連結
• 操作手冊：
• 物質安全資料表(Safety Data Sheet; SDS)：英文連結

參考規範：食品中污染物質及毒素衛生標準

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