AFLATOXIN 總黃麴毒素-定量-快篩試紙-酒精萃取-偵測儀器No. 9696

• 原理
  側流體免疫層析法 (lateral flow immunochromatographic assay)，為快篩試劑的使用原理，藉由抗體抗原間的專一性與免疫親和力做檢測，被普遍應用在、病毒感染確認、妊娠測試、警局臨檢驗毒等方面。此方法多用於定性檢測，且顯色標定物上也有許多不同的種類，目前大多數的顯色方式是利用抗體吸附在膠體金（colloid gold）來呈色。
  
   
• 結果判讀
  極限:
  偵測極限(limit of detection, LOD)： 2 ppb 
  定量極限(limit of quantification, LOQ)：3 ppb
  偵測範圍:3–100 ppb(樣品超過100ppb需重新稀釋及測量)
   
• 樣品前置處理(SAMPLE PREPARATION)
  The sample to be tested should be collected according to accepted sampling techniques (see FGIS sampling protocol or contact your NEOGEN representative). Obtain a representative sample (minimum 100 g). Grind the sample so at least 95% of the ground material passes through a 20 mesh sieve (about the particle size of fine espresso).
   
• 樣品萃取(SAMPLE EXTRACTION)
  1. If not using NEOGEN’s prepared solution, prepare a 65% ethanol solution by mixing 6.5 parts ethanol with 3.5 parts distilled or deionized water for each sample.
  2. Weigh out 10 g ± 0.1 g of sample into an extraction cup.
  3. Add 50 mL of 65% ethanol to the extraction cup.
  4. Vigorously shake, using hand or mechanical means, for 3 minutes, or blend for 1 minute.
  5. Allow the sample to settle, then filter with a filter syringe or Whatman #4 filter pa per to collect a minimum of 3 mL filtrate into a sample collection tube. Or, you may also pipette 1 mL of sample into a 2.0 mL micro-centrifuge tube, and centrifuge for 1 minute using a micro-centrifuge (approx. 2,000 x g). 
   
• 操作流程(TEST PROCEDURE)
  1. Place the appropriate number of red sample dilution cups and clear sample cups into a sample cup rack. Label cups if necessary.
  2. Add 100 µL of sample extract to each red sample dilution cup.
  3. Add 500 µL of sample diluent to the red sample dilution cup containing the sample extract. Mix by pipetting up and down 5 times.
  4. Transfer 100 µL of diluted sample extract into a new clear sample cup.
  5. Place a new Reveal Q+ for Aflatoxin test strip with the sample end down into the sample cup and set timer for 6 minutes. Ensure the test strip comes into contact with liquid and begins to wick.
  6. Remove the strip from the sample cup after it has developed for exactly 6 minutes. Immediately (within 30 seconds) read the test strip in the Raptor Solo reader.
   
• 稀釋步驟(樣品超過100ppb需重新稀釋及測量)
  Example dilution:
  1. Add 100 µL sample filtrate to a sample collection tube.  Add 100 µL 65% ethanol. Mix well.
  2. Place the appropriate number of red sample dilution cups and clear sample cups into a sample cup rack.  Label cups if necessary.
  3. Add 100 μL of diluted sample extract from step 1 to each red sample dilution cup.
  4. Add 500 μL of sample diluent to the red sample dilution cup containing the sample extract. Mix by pipetting up and down 5 times.
  5. Transfer 100 µL of sample into a new clear sample cup.
  6. Place a new Reveal Q+ for Aflatoxin test strip with the sample end down into the sample cup and set timer for 6 minutes. Ensure the test strip comes into contact with liquid and begins to wick.
  7. Remove the strip from the sample cup after it has developed for exactly 6 minutes. Immediately (within 30 seconds) read the test strip in the Raptor Solo reader.
  8. Final result displayed will need to be multiplied by 2.

• 產品說明書：連結
• 操作手冊：連結
• 物質安全資料表(Safety Data Sheet; SDS)：英文連結

參考規範：食品中污染物質及毒素衛生標準

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