Zearalenone 玉米赤黴烯酮-定量-快篩試紙-酒精萃取

• 原理
  側流體免疫層析法 (lateral flow immunochromatographic assay)，為快篩試劑的使用原理，藉由抗體抗原間的專一性與免疫親和力做檢測，被普遍應用在、病毒感染確認、妊娠測試、警局臨檢驗毒等方面。此方法多用於定性檢測，且顯色標定物上也有許多不同的種類，目前大多數的顯色方式是利用抗體吸附在膠體金（colloid gold）來呈色。
  
  玉米赤黴烯酮(Zearalenone)又稱玉米烯酮或F-2 toxin，此毒素主要由真菌-鐮孢菌（Fusarium spp.）產生，玉米赤黴烯酮由於在結構上類似雌激素，因此能與雌激素受體結合，引發類似雌激素之效應，故此毒素主要針對動物之生殖系統產生毒性
   
• 結果判讀
  玉米極限:
  偵測極限(limit of detection, LOD)： 50 ppb 
  定量極限(limit of quantification, LOQ)：50 ppb
  
  小麥極限:
  偵測極限(limit of detection, LOD)： 25 ppb 
  定量極限(limit of quantification, LOQ)：25 ppb
  
  偵測範圍:25-1200 ppb(樣品超過1200ppb需重新稀釋及測量)
   
• 樣品準備(SAMPLE PREPARATION)
  The sample to be tested should be collected according to accepted sampling techniques (see FGIS sampling protocol or contact your NEOGEN representative). Obtain a representative sample (minimum 100 g). Grind the sample so at least 95% of the ground material passes through a 20- mesh sieve (about the particle size of fine espresso). If not using NEOGEN’s prepared solution, prepare a 65% ethanol solution by mixing 6.5 parts ethanol with 3.5 parts distilled or deionized water for each sample.
   
• 玉米樣品萃取(SAMPLE EXTRACTION)
  1. Extract at a ratio of 1 part sample to 3 parts 65% ethanol. For example, combine 10 g of ground sample with 30 mL of 65% ethanol.
  2. Vigorously shake, using hand or mechanical means (250 rpm) for 3 minutes, or blend for 1 minute.
  3. Allow the sample to settle, then filter at least 4 mL with a filter syringe, or Whatman No. 1 filter paper. Alternatively, pipette sample into a 2.0 mL microcentrifuge tube and centrifuge for 30 seconds.
  4. The sample is now ready for testing.
   
• 玉米樣品萃取(SAMPLE EXTRACTION)— 50 g (FGIS Method)
  1. Combine 50 g of ground sample with 150 mL of 65% ethanol.
  2. Vigorously shake, using hand or mechanical means (250 rpm) for 3 minutes.
  3. Allow the sample to settle for 1 minute, then filter at least 3-5 mL with a filter syringe
  4. The sample is now ready for testing.
   
• 玉米樣品操作流程(TEST PROCEDURE)
  1. Place the appropriate number of red sample dilution cups and clear sample cups into a sample cup rack. Label cups if necessary
  2. Add 100 µL of sample extract to the red sample cup.
  3. Add 200 µL of sample diluent to the red dilution cup with the sample extract. Mix by pipetting up and down 5 times.
  4. Transfer 100 µL of diluted sample extract into a new clear sample cup.
  5. Place a new Reveal Q+ for Zearalenone test strip with the sample end down into the sample cup and set timer for 6 minutes. Ensure the test strip comes into contact with liquid and begins to wick.
  6. Remove the strip from the sample cup after it has developed for 6 minutes and read immediately (within 30 seconds).
  7. Remove promptly at 6 minutes and interpret results using the Raptor
   
• 玉米樣品稀釋步驟(Dilution Procedure) (樣品超過1200ppb需重新稀釋及測量)
  1. Add 100 µL sample filtrate to a sample collection tube.
  2. Add 200 µL 65 % ethanol solution to the sample collection tube. Mix well.
  3. Place the appropriate number of red sample dilution cups and clear sample cups into a sample cup rack. Label cups if necessary
  4. Add 100 µL of diluted sample extract (from step 2) to the red sample cup.
  5. Add 200 µL of sample diluent to the red dilution cup with the sample extract. Mix by pipetting up and down 5 times.
  6. Transfer 100 µL of diluted sample extract into a new clear sample cup.
  7. Place a new Reveal Q+ for Zearalenone test strip with the sample end down into the sample cup and set timer for 6 minutes. Ensure the test strip comes into contact with liquid and begins to wick.
  8. Remove the strip from the sample cup after it has developed for 6 minutes and read immediately (within 30 seconds).
  9.  Remove promptly at 6 minutes and interpret results using the Raptor
  Final result displayed will need to be multiplied by 3.
   
• 小麥樣品萃取(SAMPLE EXTRACTION)
  1. Extract at a ratio of 1 part sample to 5 parts 65% ethanol. For example, combine 10 g of ground sample with 50 mL of 65% ethanol.
  2. Vigorously shake, using hand or mechanical means (250 rpm) for 3 minutes, or blend for 1 minute.
  3. Allow the sample to settle, then filter at least 4 mL with a filter syringe, or Whatman No. 1 filter paper. Alternatively, pipette sample into a 2.0 mL microcentrifuge tube and centrifuge for 30 seconds.
  4. The sample is now ready for testing.
   
• 小麥樣品萃取(SAMPLE EXTRACTION)— 50 g (FGIS Method)
  1. Combine 50 g of ground sample with 250 mL of 65% ethanol.
  2. Vigorously shake, using hand or mechanical means (250 rpm) for 3 minutes.
  3. Allow the sample to settle for 1 minute, then filter at least 3–5 mL with a filter syringe
  4. The sample is now ready for testing. NOTE: when the test is complete, multiply the results by 1.66 for wheat.
   
• 小麥樣品操作流程(TEST PROCEDURE)
  1. Place the appropriate number of red sample dilution cups and clear sample cups into a sample cup rack. Label cups if necessary
  2. Add 100 µL of sample extract to the red sample cup.
  3. Add 200 µL of sample diluent to the red dilution cup with the sample extract. Mix by pipetting up and down 5 times.
  4. Transfer 100 µL of diluted sample extract into a new clear sample cup.
  5. Place a new Reveal Q+ for Zearalenone test strip with the sample end down into the sample cup and set timer for 6 minutes. Ensure the test strip comes into contact with liquid and begins to wick.
  6. Remove the strip from the sample cup after it has developed for 6 minutes and read immediately (within 30 seconds).
  7. Remove promptly at 6 minutes and interpret results using the Raptor
   
• 小麥樣品稀釋步驟(Dilution Procedure) (樣品超過1200ppb需重新稀釋及測量)
  1. Add 100 µL sample filtrate to a sample collection tube.
  2. Add 200 µL 65 % ethanol solution to the sample collection tube. Mix well.
  3. Place the appropriate number of red sample dilution cups and clear sample cups into a sample cup rack. Label cups if necessary
  4. Add 100 µL of diluted sample extract (from step 2) to the red sample cup.
  5. Add 200 µL of sample diluent to the red dilution cup with the sample extract. Mix by pipetting up and down 5 times.
  6. Transfer 100 µL of diluted sample extract into a new clear sample cup.
  7. Place a new reveal Q+ for Zearalenone test strip with the sample end down into the sample cup and set timer for 6 minutes. Ensure the test strip comes into contact with liquid and begins to wick.
  8. Remove the strip from the sample cup after it has developed for 6 minutes and read immediately (within 30 seconds).
  9. Remove promptly at 6 minutes and interpret results using the Raptor
  Final result displayed will need to be multiplied by 3.

• 產品說明書：連結
• 操作手冊：連結
• 物質安全資料表(Safety Data Sheet; SDS)：中文連結、英文連結

參考規範：食品中污染物質及毒素衛生標準