- 成分
Enzymatic Digest of Casein | 5.0 g/L |
Enzymatic Digest of Animal Tissue | 5.0 g/L |
Beef Extract | 5.0 g/L |
Dextrose | 5.0 g/L |
Disodium Phosphate | 4.0 g/L |
Ferrous Sulfate | 0.3 g/L |
Bismuth Sulfite Indicator | 8.0 g/L |
Brilliant Green | 0.025 g/L |
Agar | 20.0 g/L |
Final pH: 7.7 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
- 配製方法
1. Suspend 52 g of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute. <攪拌加熱至沸騰溶解約1分鐘,不須高壓滅菌>
3. Mix thoroughly to obtain a uniform suspension prior to dispensing.
4. Freshly prepared Bismuth Sulfite Agar is very selective, therefore suitable for heavily contaminated samples. However, it is not recommended to store prepared Bismuth Sulfite Agar at 4°C for longer than 2 days; after 3 days of storage the medium may change to a green color with a reduction in selectivity of competing microorganisms and an increased potential for the inhibition of Salmonella colonies.
<培養基以當天使用最佳,最多不得超過48小時,且應貯存於暗處>