- 成分
| Beef Extract | 1.0 g/L |
| Peptone | 10.0 g/L |
| D-Mannitol | 10.0 g/L |
| Sodium Chloride | 10.0 g/L |
| Phenol Red | 0.025 g/L |
| Agar | 15.0 g/L |
Final pH: 7.2 ± 0.2 at 25°C
The formula may be adjusted and/or supplemented as required to meet performance specifications.
- 補充劑成分
1️⃣NCM4017-Egg Yolk Emulsion - 50% (每瓶100ml)
NCM4017-100 Egg Yolk 50ml Distilled Water 50ml
2️⃣NCM4032-Polymyxin B Supplement
✅(每瓶需用5ml無菌去離子水進行溶解)
✅(配置1公升的MYP需加入2瓶)
✅Polymyxin B 每公升含量 : 100,000 IU/L
Per 500 mL Vial
NCM4032-0.5Polymyxin B 50,000 IU
- 配製方法
1. Suspend 46 grams of the medium in 950 mL of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45 - 50°C and supplement per method below.
5. Aseptically add 50 mL of NCM4017* Egg Yolk Emulsion and 2 vials of NCM4032-0.5* Polymyxin B
*Supplement each reconstituted using 5 mL of sterile deionized/RO water.*
Preparation per FDA BAM (較符合台灣的公告法)
1. Suspend 46 grams of the medium in 900 mL of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45 - 50°C and supplement per method below.
5. Aseptically add 50 mL of NCM4017* Egg Yolk Emulsion(50%) and 2 vials of NCM4032-0.5* Polymyxin B
*Supplement each reconstituted using 5 mL of sterile deionized/RO water.*
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.
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